Knockdown of astrocyte elevated gene-1 inhibits proliferation and enhancing chemo-sensitivity to cisplatin or doxorubicin in neuroblastoma cells

Knockdown of astrocyte elevated gene-1 inhibits proliferation and enhancing chemo-sensitivity to cisplatin or doxorubicin in neuroblastoma cells

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Abstract Background Astrocyte Dining Room Tables elevated gene-1 (AEG-1) was originally characterized as a HIV-1-inducible gene in primary human fetal astrocyte.Recent studies highlight a potential role of AEG-1 in promoting tumor progression and metastasis.The aim of this study was to investigate if AEG-1 serves as a potential therapeutic target of human neuroblastoma.Methods We employed RNA interference to reduce AEG-1 expression in human neuroblastoma cell lines and analyzed their phenotypic changes.

Results We found that the knockdown of AEG-1 expression in human neuroblastoma cells significantly inhibited cell proliferation and apoptosis.The specific downregulation induced cell arrest in the G0/G1 phase of Right Pulse Grip Speed Control cell cycle.In the present study, we also observed a significant enhancement of chemo-sensitivity to cisplatin and doxorubicin by knockdown of AEG-1.Conclusion Our study suggests that overexpressed AEG-1 enhance the tumorogenic properties of neuroblastoma cells.

The inhibition of AEG-1 expression could be a new adjuvant therapy for neuroblastoma.